Plasmid Miniprep using PEG6000

1, Spin down 2~5 ml E coli cells.
2. Add 0.25 ml P1.
3, Resuspend by votexing.
4, Add 0.25 ml P2. Lyse for 2~5 min.
5. Add 0.35 ml N3, mix
6. Spin for 2~5 min.
7. Transfer supernatant to another clean 1.5 ml microcentrifuge tube.
8. Add 160 ul 50% PEG6000, 125 ul of 4M NaCl, mix
9. Spin for 3~5 min, dump off liquid.
10. Wash with 100 ul of 70% ethanol.
11. Remove residual ethanol
12. Dissolve pellet in 50 ul EB.