2381 2383 2386 expression screening 2375 purification

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Protein Expression

[edit]

2014/11/24
SHANYI

RTENOTITLE

Nico21(DE3)plysN 0.2mM IPTG RT induce overnight




MW, PI
Expression
1 2381
pT7A3-TRX-Tat-NLS-iFlp-6H
9.32 / 66.1
supernatant

2 2381
pT7A3-TRX-Tat-NLS-iFlp-6H
9.32 / 66.1
pellet
Y
3 2383
pT7A3-6XHis-SMT3-Tat-NLS-iFlp-6H
9.49 / 65.0
supernatant

4 2383
pT7A3-6XHis-SMT3-Tat-NLS-iFlp-6H
9.49 / 65.0
pellet
Y
5 2386
pET30B-T4-uvsX 

supernatant

6 2386
pET30B-T4-uvsX 

pellet

7
PageRuler Plus



8 2375
pT7A3-MBP-TRX-6H

supernatant

9 2375
pT7A3-MBP-TRX-6H

supernatant

10 2375
pT7A3-MBP-TRX-6H

pellet
Y
11 2375
pT7A3-MBP-TRX-6H

pellet
Y
12 2375
pT7A3-MBP-TRX-6H

pellet
Y