Fast Ligase Buffer
2X Quick Ligation Reaction Buffer:
132 mM Tris-HCl
20 mM MgCl2
2 mM dithiothreitol
2 mM ATP
15% Polyethylene glycol (PEG 6000)
pH 7.6 @ 25°C
2X Quick Ligation Reaction Buffer:
132 mM Tris-HCl
20 mM MgCl2
2 mM dithiothreitol
2 mM ATP
15% Polyethylene glycol (PEG 6000)
pH 7.6 @ 25°C