344-DsbA-PET30b
Cloning of DsbA by Gene Synthesis
Purpose: to clone DsbA-PET30b
Date: 2011-9-27
Notebook: luyingying
Page:
Clone ID:344
Experimenters: lu yingying
Result
template:gene synthesis DsbA1 to DsbA18
PrimerF:DsbA1,PrimerR:DsbA18
digest result:Nde1+Kpn1
Band 1,2 show DsbA digest with 10*fast digetion buffer,Band 3 shows PET30b digest with 10*fast digetion buffer
Band 4 show DsbA digest with 10*NEB(1) buffer,band 5 shows PET30b digest with 10*NEB(1) buffer
no result
Gene Synthesis
Colony Screening
Restart:
Date: 2011-10-4
Notebook: luyingying
Page:
Clone ID:344
Experimenters: lu yingying
Result
All band show DsbA PCR product
template:gene synthesis DsbA1 to DsbA18
PrimerF:DsbA1,PrimerR:DsbA18
digest result:Nde1+Kpn1
Band 1 shows DsbA digest with 10*fast digetion buffer,Band 2 shows PET30b digest with 10*fast digetion buffer
344,1-6 bands show digest result: no insert