167 pT7A3-MBP-TRX-6H

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to remove NcoI site,

167 two BsaI sites.
Clone annealled oligos into Xba1-Nco1 digested parental vector 2375
2094-T
CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAT
TTTATTAAAACAAATTGAAATTCTTCCTCTATATAGTAC
2094-B
CATGATATATCTCCTTCTTAAAGTTAAACAAAATTATTT

167 #2 is good, sequenced

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Cloning