165 pT7A3-MBP-(TEV)

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Redo

Clone annealled oligos into Xba1-Nco1 digested  2369 pT7A3-lc-MBP-(TEV)
2094-T
CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAT
    TTTATTAAAACAAATTGAAATTCTTCCTCTATATAGTAC
2094-B
CATGATATATCTCCTTCTTAAAGTTAAACAAAATTATTT

2094 should not grow on Kan plate, but there are colonies. so it is contaminated clone,

1/2/2019, digestion NcoI+ Xho1

http://www.excellgen.net/eln/images/8/8e/165_168_digestion.JPG

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Cloning