172 pT7A3-MBP-(Tev)-GST-(3C)-sfGFP

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Target ID	Enzymes	Insert from	Insert Size	Vector
171	Xba1+ NcoI	168 pT7-MBP-(tev)-GST-(3c)-sfGFP	1930	166 pBad-MBP-(Tev)-GST-(3C)-sfGFP
172	XbaI + Nco1	168 pT7-MBP-(tev)-GST-(3c)-sfGFP	1930	161 pT7A3-MBP-(Tev)-GST-(3C)-sfGFP

Plasmid digestion, 1/5/19

168 could have problem

171 could have problem

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Cloning