Preparation of Products from Stock
T3 DNA Ligase
[edit]40 ul stock, + 310 ul storage buffer--> 350 ul, 2.12 mg/ml
T7 DNA Ligase
[edit]270 ul stock + 170 ul storage buffer --> 2.5 mg/ml
4X Ligase Buffer, 50 ml
[edit]| 250 mM TrisHCl, pH 7.6 | 9.4 ml 1 M Tris-HCl | 3.25 ml 1M TrisMa |
| 40 mM MgCl2 | 2 ml 1 M MgCl2 | |
| 4 mM DTT | 200 ul 1 M DTT | |
| 4 mM ATP | 125 mg ATP, MW 551 | |
| 30% PEG 6000 | 15 g | |
| H2O | ddH2O to 50 ml |