CETP VLP Expression Screening
expression Screening in urea soluble fraction (inclusion body)
| Lane | ID | Name | Host | pI/MW | Induction Condition | Expressed? |
|---|---|---|---|---|---|---|
| M | Protein Marker | PageRuler Plus | 10 15 25red 35 55 70red 100 130 250 | |||
| 1 | 2557 | pET28a-Qbeta-CETP-N1-Avitag-6His | Nico(DE3)pLysN | 7.86 / 19.6 | 37 oC for 4 hours | yes, IB |
| 2 | 2558 | pET28a-Qbeta-CETP-N2-Avitag-6His | Nico(DE3)pLysN | 6.43 / 20.2 | 37 oC for 4 hours | yes, IB |
| 3 | 2559 | pET28a-Qbeta-CETP-N3-Avitag-6His | Nico(DE3)pLysN | 7.13 / 20.2 | 37 oC for 4 hours | yes, IB |
| 4 | 2555 | pET28a-Qbeta-CETP-N13-Avitag-6His | Nico(DE3)pLysN | 7.87 / 22.4 | 37 oC for 4 hours | yes, IB |
| 5 | 2556 | pET28a-Qbeta-CETP-N12-Avitag-6His | Nico(DE3)pLysN | 6.75 / 22.6 | 37 oC for 4 hours | yes, IB |
| 6 | 2560 | pET28a-CETP-N1-AP205 | Nico(DE3)pLysN | 8.98 / 17.8 | 37 oC for 4 hours | yes, IB |
| 7 | 2561 | pET28a-CETP-N2-AP205 | Nico(DE3)pLysN | 7.06 / 18.3 | 37 oC for 4 hours | yes, IB |
| 8 | 2562 | pET28a-CETP-N3-AP205 | Nico(DE3)pLysN | 8.65 / 18.3 | 37 oC for 4 hours | yes, IB |
| 9 | 2563 | pET28a-CETP-N12-AP205 | Nico(DE3)pLysN | 7.85 / 20.6 | 37 oC for 4 hours | yes, IB |
| 10 | 2564 | pET28a-CETP-N13-AP205 | Nico(DE3)pLysN | 8.95 / 20.6 | 37 oC for 4 hours | yes, IB |
| 11 | 971 | pET28a-SpyCatcher-AP205cp3 | Nico(DE3)pLysN | 6.50 / 26 | 37 oC for 4 hours | yes, IB |
| 12 | 2565 | pET29b-MT-MMLV | Nico(DE3)pLysN | 9.08 / 79.6 | 37 oC for 4 hours | yes, more as inclusion body |
| 13 | 2566 | pET29a-Qbeta-SpyCatcher-Avitag-6His | Nico(DE3)pLysN | 6.20 / 27.7 | 37 oC for 4 hours | yes, IB |
| 14 | 2567 | pET29b-RT-Pol | Nico(DE3)pLysN | 6.80 / 101.5 | 37 oC for 4 hours | yes, not much |