Protein Structure Prediction

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The PCV2 capsid protein (CP) structure was generated with I-TASSER [32], [33]. The model was built using the PCV2 CP sequence (NCBI/GenBank accession number: AAC35310). The following experimentally determined templates were used to thread the PCV2 CP structure: PDB ID codes: 2EIG (Lotus tetragonolobus seed lectin), 1V6I (Peanut lectin), 1C8N (Tobacco necrosis virus CP), 1NT0 (CUB1-EGF-CUB2 region of mannose-binding protein), 1NG0 (Cocksfoot mottle virus CP), 1JOD (pituitary adenylatecyclase-activating polypeptide), 1E4B (L-fuculose-1-phosphate aldolase). A PCV2 CP pentamer was created with Symmdock [34] for graphical epitope visualization