Multitag iFlp Expression Screening

Protein Expression Screening

Cells: Nico21(DE3)pLysN, 15 ml LB/KC, with 1 X5052

#	Clone ID	Name	Induction	His Tag	pI MW	Expression	Anomaly observation
1	2596	pT7A30-D7324-6H-CCMV	1 mM IPTG, 18 oC o/n		9.89 / 23.4
2	2586	pET28a-6H-D7324-AP205	1 mM IPTG, 18 oC o/n		9.88 / 17.6		most cells lysed
3	2595	pDB.His.GST-D7324	1 mM IPTG, 18 oC o/n		6.90 / 31		most cells lysed
4	2594	pDB.His.GST-SpyCatcher	1 mM IPTG, 18 oC o/n		5.75 / 39.3		most cells lysed
5	2593	pDB.His.GST-SpyCatcher-AP205	1 mM IPTG, 18 oC o/n		5.99 / 53.6		most cells lysed
6	2585	pT7A3-TRX-multi-Tags	1 mM IPTG, 18 oC 4h		4.67 / 49.7		most cells lysed
7	2587	pT7A3-GST-multi-Tags	1 mM IPTG, 18 oC o/n		4.97 / 63.9		most cells lysed
8	2604	pCold-MBP-multitag	1 mM IPTG, 18 oC o/n		4.88 / 80.6
9	2580	pET28a-Qbeta-Tat-6His	1 mM IPTG, 18 oC o/n		10.35 / 18.7		most cells lysed
10	2579	pET28a-6H-Tat-AP205	1 mM IPTG, 18 oC o/n		10.54 / 18.3		most cells lysed
11	129	pT7A30-TthMuts	1 mM IPTG, 18 oC o/n		5.87 / 91.8		most cells lysed
12	136	pL6H-Tat-Cre	1 mM IPTG, 18 oC o/n		10.30 / 42.7
13	135	pQE-80L-HTNCre	1 mM IPTG, 18 oC o/n		10.30 / 42.8
14	170	pT7-MBP-(tev)-GST-(3c)-Tat-NLS-iFLP-6H	1 mM IPTG, 18 oc 4 hours		8.64 / 123.3
15	169	pBad-MBP-(Tev)-GST-(3C)-Tat-NLS-iFLP-6H	ara, 0.2% 1 mM IPTG, 18 oC o/n		8.64 / 123.4		most cells lysed
16	164	pT7A3-MBP-(Tev)-GST-(3C)-Tat-NLS-iFLP-6H	1 mM IPTG, 37 oC 4h		8.64 / 123.4
17	162	pCold-MBP-(Tev)-GST-(3C)-Tat-NLS-iFLP-6H	1 mM IPTG, 18 oC o/n		8.64 / 123.4
18	211	pDBHisMBP-TaqMuts	1 mM IPTG, 18 oC o/n		5.40 / 134
19	941	pT7A3-pMBP-T7-Endo-1	1 mM IPTG, 37 oC 4h		7.70 / 63.4
20	2619	pT7A3-pMBP-T7EndoI-PA/A	1 mM IPTG, 37 oC 4h		7.35 / 64.4

S: soluble, P: inclusion body

M: PageRuler Plus, 10, 15, 25, 55, 70,100, 130, 250

9/26/19

transferred to membrane

sup