2625
Expression Screening
Expression host: Nico21(DE3)pLysN, 0.5 M IPTG
| Lane | ID | Name | Sample | Induction | Expression |
| M | PageRuler Plus | ||||
| 1 | 2628 |
pET28b-hFth-iFlp |
from 0.5 ml of 8 L exp, 37 oC 5 hours, S | 0.5 mM IPTG | |
| 2 | 2628 |
from 15 ml small scale, 18 oC induction, S | 1 mM | ||
| 3 | 2628 |
from 15 ml small scale, 18 oC induction, P | 1 | ||
| 4 | 2628 |
from 15 ml small scale,37 oC induction, S | 1 | ||
| 5 | 2628 |
from 15 ml small scale,37 oC induction, P | 1 | ||
| 6 | 2626 | pCold-GST-CYFRA 21-1-avitag-6H |
15 ml small scale, 18 oC induction, S |
1 | |
| 7 |
2626 + 2625 |
pCold-GST-CYFRA 21-1-avitag-6H + pCold-GST-Ac-LysargiNase |
from 2626 15 ml small scale, 18 oC induction, P + from 2625 15 ml small scale, 18 oC induction, S |
1 | |
| 8 | 2625 | pCold-GST-Ac-LysargiNase |
15 ml small scale, 18 oC induction, P |
1 | |
| M | |||||
| 9 |