Protein Expression Tasks
| Status | Clone ID | Name | MW, PI |
Host |
Antibiotics |
Inducer |
Method/Protocol |
Date |
Note |
| 1078 |
pET28b-scIHF2 |
9.60 / 22.8 |
no expression before EG-39, 1 F11, 1 G10 | ||||||
| 1203 | pET42b-scIHF2 |
8.38 / 54.2 |
EG-39, no expression before, 9 B2 | ||||||
| 1074 |
pET28b-IHFab |
9.20 / 25.3 |
unknow expression, 1 A8, 1 B9 | ||||||
| 1149 |
pT7A3E-6H-HC-Xis |
10.93 / 10 |
EG-37, inclusion body, 2 I7 | ||||||
| 1186 |
pDB.His.MBP-Xis |
6.62 / 52.1 |
EG-37, low expression http://www.excellgen.net/eln/index.php/1143-1151-1186-screening, 2 F4 | ||||||
| 407 | pET28b-Xis |
11.34 / 10.7 |
EG-37 | ||||||
| 406 |
pET28b-Int |
9.53 / 42.5 |
don't know if there is any expression, 38 D3 | ||||||
| 1185 |
pDB.His.MBP-Int |
7.33 / 83.7 |
2 F3 | ||||||
| 3105 |
pET28b-AaLS |
||||||||
| 3127 |
pET28b-qBeta-qBeta-6XHis |
||||||||
| 3098 |
pET28b-AP205 |
need to check the expression of BamH1 digested/religated clone | |||||||
| 812 |
pET30b-phoA |
||||||||
| 938 | PNGase F, | ||||||||
| 939 | Tte-Exo III | ||||||||
| 942 | VVN endonuclease | ||||||||
| 3122 | Crm197, secreted | ||||||||
| 931 | PAK16D ligase | ||||||||
| done | 945 |
pT7A30-pMBP |
|||||||
| done | 947 |
pT7A30-6His-pMBP |
|||||||
| 944 | pET30b-pfu-exo- |
||||||||
| 824 |
pET28b-VVTopo |
||||||||
| 3107 pFastbac-Cel1 |
|||||||||
| 1868 |
pT7A30-fMMLV |
||||||||
| 1747 |
pT7A30-iMMLV |
||||||||
| 1483 |
pT7A3-T4_DNA_Ligase |
||||||||
| 927 | pET28b-Cid1 | no expression | |||||||