Gel Staining

1. Carefully transfer gel to a transparent container, such as pipette box .

2. Add ~50~100 ml ddH2O, microwave heat to boil, leave it  in hot water for 1 min. 

3. Decant water.

4. Add ~50~100 ml Excellgen staining solution

5. Microwave to boil, leave the gel and container in microwave oven for 30 min.

6. Do not discard protein stain, it is very expensive to make, transfer the stain to its original container.

7. Rinse the gel with ddH2O

8. Add 50~100 ml ddH2O

9. Microwave heat to biol.

10. Decant hot H2O.

11 Add large amount of ddH2O, do not boil again, the protein bands should be visible now.

12. Destain the gel by soaking in water,  change H2O several times.