Packagingmix optimization 3
Notebook #:
Page #: 001431
2 days after transfection
--Paul.xu 10:56, 30 January 2011 (EST)
3 days after transfection
4 days after transfection
--Ztao 10:10, 3 February 2011 (EST)
5 days after transfection
--Paul.xu 09:42, 4 February 2011 (EST)
1 day after infection
--Paul.xu 14:15, 1 February 2011 (EST)
2 days after infection
--Ztao 10:16, 3 February 2011 (EST)
3 days after infection
--Paul.xu 09:48, 4 February 2011 (EST)
4 days after infection --Paul.xu 14:34, 5 February 2011 (EST)
5 days after infection
--Paul.xu 10:32, 6 February 2011 (EST)
| Vector |
Packaging mix |
Transfection Reagent | |
| 1 |
- |
- |
- |
| 2 |
- |
Sigma |
PolyExpress |
| 3 |
TurboGFP |
Sigma |
PolyExpress |
| 4 |
TurboGFP |
1:1 |
PolyExpress |
| 5 |
TurboGFP |
1:3 |
PolyExpress |
| 6 |
TurboGFP |
1:5 |
PolyExpress |
| 7 |
TurboGFP |
1:10 |
PolyExpress |
| 8 |
TurboGFP |
Sigma |
GeneExpresso |
| 9 |
TurboGFP |
1:3 |
GeneExpresso |
| 10 |
pSMPUW-EGFP |
1:3 |
PolyExpress |
| 11 |
pSMPUW-EGFP |
Sigma |
PolyExpress |
| 12 |
pSMPUW-EGFP |
Sigma |
GeneExpresso |
GeneExpress: 1.2 ul/50ul
PolyExpress: 1.2 ul/50ul
TurboGFP:0.2 ug (1ul of 200 ng/ul)
pSMPUW-EGFP: 0.2 ug (1ul of 200 ng/ul)
Packaging mix: 2 ul (Sigma or homemade, pVSVG/pGag-Pol)
--Ztao 17:00, 28 January 2011 (EST)