2718
plasmid in vector pCDNA5-frt, digest with Nhe1-Not1 at 37 oC for 20 min
plasmid in vector pCDNA5-frt, digest with Nhe1-Xho1 at 37 oC 1h
plasmid in vector pCDNA5-frt, digest with Nhe1-Xho1 at 37 oC o/n
plasmid in vector pCDNA5-frt, digest with Nhe1-Not1 at 37 oC for 20 min
plasmid in vector pCDNA5-frt, digest with Nhe1-Xho1 at 37 oC 1h
plasmid in vector pCDNA5-frt, digest with Nhe1-Xho1 at 37 oC o/n