2763

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PCR from genomic DNA using superhifi II enzyme mix

6/6/2020

PCR again from genomic DNA

6/10/2020

digestion miniprep DNA with Xho1

subclone 2763-pMiniT2 to 2660-pET29 with NdeI+XhoI

20200616 2763 pJet NdeI+XhoI in Cutsmart, 37oC, 1hour

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Cloning