2773

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Cloning

PCR using Taq

PCR using Thermofisher SuperFi II

PCR

PCR from genomic DNA using superhifi II enzyme mix

6/6/2020

Miniprep Digestion

06092020

6/10/2020

digestion miniprep DNA with Xho1

2773 in pJet1.2

digested with Xba1-Xho1

2696 Nco1-Xho1 digested vector is for 2772

20200616 2773 pJet NdeI+XhoI in Cutsmart, 37oC, 1hour

2773 gibson

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Cloning