6X Loading Dye

1% Cresol Red Dye Stock yield 50 ml

Add 500 mg to 50 ml of ddH2O in a 50 ml tube or bottle

Shake to dissolve,Store at room temperature

Cresol Red Loading Dye containing 0.02% Cresol Red

yield 100 ml of working solution
Add 30 g of glycerol to ~70 ml of ddH2O
Shake to dissolve
Add 2 ml of 1% Cresol Red Dye or amaranth, adjust to 100 ml
Shake tube to mix. Store at 4°C

Protocol 2

• 300 ml glycerol (30%)
• 2.5 g bromophenol blue or Cresol Red (0.25%)
• dH₂O to 1000mL

10X Xylene Cyanol/Bromophenol Blue DNA loading buffer recipe

• 0.025 g of Xylene Cyanol FF
• 0.025 g of Bromophenol Blue0.025 g cresol red (optional)
• 1.25 ml of 10% SDS (optional)
• 4 ml 0.5 M EDTA (optional)
• 2 ml 1 M Tris-Cl pH 7.6-8.0 (optional)
• 12.5 ml of glycerol
• water to 20 ml Store sucrose buffers at 4 or -20C.