359-pET30-pMBP
Cloning of 359-pET30-pMBP
Purpose: to clone pET30-pMBP by PCR
Date: 2011-10-4----
Notebook:Lu yingying
Page:
Clone ID:359
Experimenters: lu yingying
Results
PCR product of pMBP
template: 424-pDEST-periHisMBP
primer:periMBP-F + 10% periMBP-R1 + periMBP-R2
25 cycles
digest result:NdeI + NcoI
band 1 shows pMBP digest result, band 2 shows PET30b digest result
359,1-6 bands show digest result: no insert
Cloning of 359-pET30-pMBP
Purpose: to clone pET30-pMBP by PCR
Date: 2011-10-17----
Notebook:Lu yingying
Page:
Clone ID:359
Experimenters: lu yingying
Results
PCR product of pMBP
template: 424-pDEST-periHisMBP
primer:periMBP-F + 10% periMBP-R1 + periMBP-R2
25 cycles
digest result:NdeI + NcoI
digest result:NdeI + NcoI
ligation RT overnight
Cloning of 359-pET30-pMBP
Purpose: to clone pET30-pMBP by PCR
Date: 2011-10-19----
Notebook:Lu yingying
Page:
Clone ID:359
Experimenters: lu yingying
Results
digest result:NdeI + NcoI NEB enzyme for 2 hours
Date: 2011-10-25----
Notebook:Lu yingying
Page:
Clone ID:359
Experimenters: lu yingying
Results
PCR product of pMBP
template: 424-pDEST-periHisMBP
primer:periMBP-F + 10% periMBP-R1 + periMBP-R2
25 cycles
10-19 PCR product 10ul left
digest result:NdeI + NcoI
359 digest detect: NdeI + NcoI
Date: 2011-11-4----2011-11-7
Notebook:Lu yingying
Page:003966----003971
Clone ID:359
Experimenters: lu yingying
Results
PCR product of pMBP
template: 424-pDEST-periHisMBP
primer:periMBP-F + 10% periMBP-R1 + periMBP-R2
25 cycles
10-19 PCR product 10ul left
digest result:NdeI + NcoI
5 clonies digest detect:NdeI + NcoI ,need to sequence to detect