Transfection reagents comparison 1
Notebook: Zhen Tao #100
Page: 001913
1 day after transfection
2 days after transfection
--Paul.xu 14:12, 5 February 2011 (EST)
3 days after transfection
--Paul.xu 10:37, 6 February 2011 (EST)
4 days after transfection
--Paul.xu 09:25, 7 February 2011 (EST)
5 days after transfection
--Paul.xu 09:54, 8 February 2011 (EST)
| well number | transfection reagent | amount of TR being used (ul) | DNA(PSMPUW-CMV-EGFP) (ug) |
| 1 | LPEI25 | 1 | 1 |
| 2 | LPEI25 | 3 | 1 |
| 3 | BPEI25 | 1 | 1 |
| 4 | BPEI25 | 3 | 1 |
| 5 | PEIMAX | 1 | 1 |
| 6 | PEIMAX | 3 | 1 |
| 7 | PolyExpress | 3 | 1 |
| 8 | GeneExpresso | 3 | 1 |
| 9 | GeneExpresso Plus | 3 | 1 |
| 10 | Proteinone | 3 | 1 |
| 11 | Proteinone | 6 | 1 |
| 12 | GeneExpresso 8000 | 3 | 1 |
1) Add transfection reagent to500 ul DMEM----> wrong
2) Add DNA to 500 ul DMEM---> wrong
3) Add transfection reagent in step 1) to DNA (step 2), mix well and keep for 20 min at room temperature
4) Add the mixture in step 3 to cells in 12 well plate.
--Ztao 09:29, 4 February 2011 (EST)