Synthesis ABOL CBA EDA

1. N’-cystaminebisacrylamide (CBA) + ABOL   in methanol / water (4/1) at 45° C for six days.

2. Add EDA and 45 oC for two days.

3. Add excess of EDA.

4. Purify by ultrafiltration (MWCO 1000, pH 5).

5. Filter through a 0.45 ÎŒm filter, recover by  lyophilization.