500-PET30b-MMLV-C

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Cloning of PET30b-MMLV-C

Purpose: to clone PET30b-MMLV-Cby PCR

Date: 2011-12-6

Notebook:Lu yingying

Page:

Clone ID:500

Experimenters: lu yingying
Results

457+471+477+500 PCR 12-5-2011.JPG

Gene synthesis of fragment 1,MMLV-31 to MMLV-52


digest with Nco1 + Xho1, clone into Nco1 + Xho1 digested pET3ob


digest detect: Nco1 + Xho1