457-PET28b-CymR

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Cloning of 457-PET28b-CymR

Purpose:  gene synthesis  to cloning of 457-PET28b-CymR

Date: 2011-11-28----

Notebook:Lu yingying

Page:

Clone ID:457

Experimenters: lu yingying
Results


Gene synthesis

template: CymR1 to CymR18 mixture

 CymR 1 and CymR18 as primers


digest:BamH1+ Xho1

band 1 show insert ,band 2 show PET28b vector


Date: 2011-12-6----

Notebook:Lu yingying

Page:

Clone ID:457

Experimenters: lu yingying
Results

457+471+477+500 PCR 12-5-2011.JPG

Gene synthesis

template: CymR1 to CymR18 mixture

CymR 1 and CymR18 as primers


digest:BamH1+ Xho1

band 1 show insert ,band 2 show PET28b vector


digest detect: BamH1+ Xho1

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