491-PET30-Cre
Cloning of 491-PET30-Cre
Purpose: to subclone from 298-pTriEx-HTNC to PET30b
Date: 2011-11-29----
Notebook:Lu yingying
Page:
Clone ID:491
Experimenters: lu yingying
Results
digest 298 pTriEx-HTNC with Nco1 +Xho1 --> insert, digest pET30b with Nco1 + Xho1 -->vector,
ligate and transform Top10
digest detect: Nco1 + Xho1
Cloning of 491-PET30-Cre
[edit]Purpose: subcloning of 298-pTriEx-HTNC to PET30b
Date: 2011-12-16---2011-12-21
Notebook:
Page:
Clone ID:491
Experimenters: yingying
Results
[edit]
| band 5 |
band 6 | |
| 298-pTriEx-HTNC |
PET30b | |
| digest |
Nco1 + Xho1 |
Nco1 + Xho1 |
Subcloning
[edit]Date: 2011-12-16
Colony Screening
[edit]Date: 2011-12-21
Make miniprep and digest with Nco1 + Xho1
Result intepretation/Summary
[edit]- Sequencing Result:491-3 right
Conclusion
[edit]491-3 right
--Yingying.lu 14:59, 21 December 2011 (EST)