459-PCMVK-Gluc-PKA-Alpha

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Cloning of 459-pAAV-CMV-RFP

Purpose: to PCMVK-Gluc-PKA-Alpha by PCR

Date: 2011-12-6----

Notebook:Lu yingying

Page:

Clone ID:459

Experimenters: lu yingying
Results


PCR using pLentiGP-Gluc-PKAalpha 300 as template and the following primers

Gluc-Xba1-F
atcga TCTAGA CCGGCCACCATGGGAGTCAAAG

PKA-6His-BamH1-R
(gcaaggagttttctgagttt CTC GAG CAT CAC CAT CAC CAT CAC TAA GGA TCC aaatag)

Cut PCR product with Xba1 + BamH1, clone into pCMVK-StrepII 193 (Xba1 + BamH1)


digest detect: Xba1 + BamH1
--Yingying.lu 13:08, 12 December 2011 (EST)


Date: 2011-12-20----

Notebook:Lu yingying

Page:

Clone ID:459

Experimenters: lu yingying
Results



PCR using pLentiGP-Gluc-PKAalpha 300 as template and the following primers

Gluc-Xba1-F
atcga TCTAGA CCGGCCACCATGGGAGTCAAAG

PKA-6His-BamH1-R
(gcaaggagttttctgagttt CTC GAG CAT CAC CAT CAC CAT CAC TAA GGA TCC aaatag)


Cut PCR product with Xba1 + BamH1, clone into pCMVK-StrepII 193 (Xba1 + BamH1)


digest detect: Xba1 + BamH1


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