535-pAAV-CMV-DIO-sfGFP

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Cloning of 535-pAAV-CMV-DIO-sfGFP

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Purpose: Cloning of 535-pAAV-CMV-DIO-sfGFP by genesythesis

Date: 2012-1-24--

Notebook:

Page:

Clone ID:535

Experimenters: yingying

Results

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Cloning

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Date: 2012-1-24


gene synthesis,

PCR1:DIO-sfGFP1 to DIO-sfGFP12

PCR2:DIO-sfGFP13 to DIO-sfGFP26

PCR: PCR1+PCR2 as template,

primer:sfGFP1+DIO-sfGFP26



 band 1
 band 2

 PCR product
 254 pAAV.CMV-MCS
digestion enzymes
         EcoR1 + BamH1



Colony Screening

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Date: 2012-1-30

Make miniprep and digest with  EcoR1 + BamH1


535-1,2,3,4,5,6 may right ,need to sequence




Result intepretation/Summary

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Conclusion

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