529-pLVX-mIL10-IRES-EGFP

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Revision as of 15:30, 31 January 2012 by Ruili.su (talk | contribs) (Created page with "'''Purpose:''' Subcloning of 529-pLVX-mIL10-IRES-EGFP by PCR<br> '''Date: '''2012-1-27---- '''Notebook:''' '''Page:''' '''Clone ID:529''' '''Experimenters:''' yingying ...")
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Purpose: Subcloning of 529-pLVX-mIL10-IRES-EGFP by PCR

Date: 2012-1-27----

Notebook:

Page:

Clone ID:529

Experimenters: yingying

Results

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Cloning

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Date: 2012-1-28


PCR using mIL10-BamH1-F + mIL10-Xho1-R, template is pBluescript SK-mIL10, 530




 band 1
 band2

PCR product
 pLVX-IRES-EGFP, 528
digest

                  BamH1 + Xho1



Colony Screening

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Date:2012-1-31

Make miniprep and digest with BamH1 + Xho1


clone 3 may be right, need to sequencing


 

Result intepretation/Summary

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Conclusion

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--Yingying.lu

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