252-pLenti-IRES-TFR-EGFP

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Cloning of pLenti-IRES-TFR-EGFP (252)

Purpose: to clone pLenti-IRES-TFR-EGFP by Gene Synthesis

Date: 2012-1-24 to 2012-1-27

Notebook: Xiaojing Wang

Page: 004143---004144

Clone ID: 252

Experimenters: Xiaojing Wang

Results


digest:


digest PCR product and vector with Xho1 + Mlu1, run gel

the left band shows 252 insert, the right picture shows vector (pLentiPlus, 152)

gel purify, ligation, transformation

Colony Screening




Select 5 clones to mini-prep, digested by Xho1 and Mlu1. 252-2,-3,-4,-5 may be correct. Control is 252-1.

Select 252-2 to sequence.


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