323-pLenti-EF1-MCS

Cloning of 323-pLenti-EF1-MCS

Purpose: Cloning of 323-pLenti-EF1-MCS

Date: 2011-12-28

Notebook:

Page:

Clone ID:323

Experimenters: yingying

Results

template: pPuroGreen 178

Primer-F EF1-NdeI-F agct GCTAGC c gtgaggctcc ggtgcccg
Primer-R EF1-BamH1-R (cttccatttcaggtgtcgtga GGATCC atcg) cgatGGATCCtcacgacacctgaaatggaag

	band 3	band 4
	PCR product	pLenti-Plus (152)
digest enzyme	Nde1 + BamH1

Subcloning

Date: 

Colony Screening

Date: 2012-1-3

Make miniprep and digest with Nde1 + BamH1

 
Result intepretation/Summary

Conclusion

--Yingying.lu 15:05, 29 December 2011 (EST)

Cloning of 323-pLenti-EF1-MCS

Purpose: Cloning of 323-pLenti-EF1-MCS

Date: 2012-1-4

Notebook:

Page:

Clone ID:323

Experimenters: yingying

Results

template: pPuroGreen 178

Primer-F EF1-NdeI-F agct GCTAGC c gtgaggctcc ggtgcccg
Primer-R EF1-BamH1-R (cttccatttcaggtgtcgtga GGATCC atcg) cgatGGATCCtcacgacacctgaaatggaag

	band 1	band 2
	PCR product	pLenti-Plus (152)
digest enzyme	Nde1 + BamH1

Subcloning

Date: 

Colony Screening

Date: 2012-1-6

Make miniprep and digest with Nde1 + BamH1

 
Result intepretation/Summary

Conclusion

--Yingying.lu 15:05, 29 December 2011 (EST)

Cloning of 323-pLenti-EF1-MCS

Purpose: Cloning of 323-pLenti-EF1-MCS by PCR

Date: 2012-1-26--

Notebook:

Page:

Clone ID:323

Experimenters: yingying

Results

cloning

Date: 2012-1-26

Primer-F EF1-NdeI-F agct CATATG c gtgaggctcc ggtgcccg
Primer-R EF1-BamH1-R (cttccatttcaggtgtcgtga GGATCC atcg) cgatGGATCCtcacgacacctgaaatggaag

	band 3	band 4
	PCR product	152 pLenti-plus
digestion enzymes	Nde1 + BamH1

Colony Screening

Date: 2012-1-30

Make miniprep and digest with  Nde1 + BamH1

clone 1,2,5 may be right, need to sequence

Result intepretation/Summary

• sequencing :no priming ,need to clone again
  

Cloning of 323-pLenti-EF1-MCS

Purpose: Cloning of 323-pLenti-EF1-MCS by PCR

Date: 2012-2-7--2012-2-10

Notebook:003732

Page:

Clone ID:323

Experimenters: yingying

Results

cloning

Date:2012-2-7

template:178- pGreenPuro(EF1)

Primer-F EF1-NdeI-F agct CATATG c gtgaggctcc ggtgcccg
Primer-R EF1-BamH1-R (cttccatttcaggtgtcgtga GGATCC atcg) cgatGGATCCtcacgacacctgaaatggaag

	band 1	band 2
	PCR product	152 pLenti-plus
digest enzymes	Nde1 + BamH1

Colony Screening

Date: 2012-2-10

Make miniprep and digest with Nde1 + BamH1

 no insert

Result intepretation/Summary

• <br>

Conclusion

<br>

<br>