Transfection reagents freeze-thaw each 2 weeks

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Make transfection reagent (TR,lot 20111107) to freeze-thaw each 2 weeks. lot 20111107

3 ul TR + 50ul DMEM without serum---->Mix A

1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum---->Mix B

Mix A + Mix B, incubate 20min at room temperature

Add 100 ul mixture to transfect 293T cells in 12 well-plate.


0 is control. (2011.11.7)

2 is TR frozen 2 weeks. (2011.11.21)

48 hours after transfection



--Xiaojing Wang 16:16, 23 November 2011 (EST)


0 is control. (2011.11.7)

4 is TR frozen 4 weeks. (2011.12.5)

48 hours after transfection


--Xiaojing Wang 13:52, 8 December 2011 (EST)


0 is control. (2011.11.7)

6 is TR frozen 6 weeks. (2011.12.5)

48 hours after transfection





0 is control. (2011.11.7)

9 is TR frozen 9 weeks. (2012.1.9)

24 hours after transfection


day 2 after transfection


day 3 after transfection


0 is control. (2011.11.7)

12 is TR frozen 12 weeks. (2012.2.1)

48 hours after transfection




0 is control. (2011.11.7)

15 is TR frozen 15 weeks. (2012.2.17)

4 days after transfection


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