Lentivirus QC 2-24-2012

Notebook: Xiaojing Wang

Page: 004184,004185

493 pLVXPlus2-App-IRES-mCherry

493 lentivirus production-293T in plates

Use psPAX2 (503) 15 ug + VSVG (505) 7.5 ug + DNA 22.5 ug in each plate

The Gene Expresso Max of 493 lentivirus production is Lot:DSP01042012.

2 days after transfection(2-24-2012), take out 0.5 ml medium and add to 1 well of 293T cells growing on 12 well plate (50% confuluency). Add 1000X polybrene, so the final concentration is 1X .

Incubate for 3 days, take pictures.