HiTrap Desalting
To operate the column with a syringe, connect the syringe to the column with the supplied luer adaptor.
1. Equilibrate the column with 10 ml buffer
2. Apply the sample using a 2–5 ml syringe.
The maximum recommended sample volume is 1.5 ml. Use a flow rate between 1–10 ml/min. Discard the eluted buffer from the column.
3. Change to buffer and proceed with injection.
If the sample volume is less than 1.5 ml, add buffer until a total of 1.5 ml buffer is eluted. Discard the eluted buffer.
4. Elute the high molecular weight components with the volumes listed in the following table
Collect the eluted buffer
| Load ml | Add ml | Buffer collect ml | Yield % | Salt % | Dilution factor |
| 0.25 | 1.25 | 1 | >95 | 0 | 4 |
| 0.5 | 1 | 1.5 | >95 | <0.1 | 3 |
| 1 | 0.5 | 2 | >95 | <0.2 | 2 |
| 1.5 | 0 | 2 | >95 | <0.2 | 1.3 |