Transfection reagents 2012-1-4
guanidinylation
QC result after 2 months storage at -20 oC: without DSP conjugation the transfection reagent will not work.
|
PHP-ss-DSP-guPEI18, 1:10 |
PHP-ss-DSP-guPEI18, 1:20 | PHP-ss-DSP-guPEI18, 1:30 | PHP-ss-DSP-guPEI18, 1:40 |
| PHP-ss-guPEI18, 1:10 | PHP-ss-guPEI18, 1:20 | PHP-ss-guPEI18, 1:30 | PHP-ss-guPEI18, 1:40 |
| guPEI18, 1:10 | guPEI18, 1:20 | guPEI18, 1:30 | guPEI18, 1:40 |
24 hours after transfection
48 hours after transfection
day3
Day4
day 5
Plate2
|
PHP-ss-DSP-guPEI18, 1:50
|
PHP-ss-DSP-guPEI18, 1:60 | PHP-ss-DSP-guPEI18, 1:70 | PHP-ss-DSP-guPEI18, 1:80 |
| PHP-ss-guPEI18, 1:50 | PHP-ss-guPEI18, 1:60 | PHP-ss-guPEI18, 1:70 | PHP-ss-guPEI18, 1:80 |
| guPEI18, 1:50 | guPEI18, 1:60 | guPEI18, 1:70 | guPEI18, 1:80 |
24 hours after transfection
48 hours after transfection
day3
day4
Day5
2011-1-10: Do transfection using more diluted polymer
|
PHP-ss-DSP-guPEI18, 1:80 |
PHP-ss-DSP-guPEI18, 1:100 | PHP-ss-DSP-guPEI18, 1:120 | PHP-ss-DSP-guPEI18, 1:150 |
| PHP-ss-guPEI18, 1:80 | PHP-ss-guPEI18, 1:200 | PHP-ss-guPEI18, 1:120 | PHP-ss-guPEI18, 1:150 |
| control, 11-10-2011 | control, 11-10-2011 |
