Lentivirus Concentration poly-L-lysine
1. Make poly-L-lysine hydrobromide (MW 27,400, Sigma), 100 mg/ml with phosphate-buffered saline (PBS)
2. Add 0.005% of PLL to lentivirus medium and incubate for 30 min for at 4°C
3. Spin at 4000 g for 1 h
1. Make poly-L-lysine hydrobromide (MW 27,400, Sigma), 100 mg/ml with phosphate-buffered saline (PBS)
2. Add 0.005% of PLL to lentivirus medium and incubate for 30 min for at 4°C
3. Spin at 4000 g for 1 h