1022-pET28b-Tth-Ligase
Cloning of 1022-pET28b-Tth-Ligase
[edit]Purpose: Cloning of 1022-pET28b-Tth-Ligase
Date: 2012-4-5--2012-4-10
Notebook:0
Page:
Clone ID:1022
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-4-5
PCR using Tth genomic DNA
Tth-Lig-Nde1-F G3 atcgcgCATATGaccctggaagaggcgaggaag
Tth-lig-EcoR1-R G4 ctctgtGAATTCttagacgagctcctccgccttc
| band 1 |
band 2 | |
| PCR product |
pET28b | |
| enzymes |
Nde1+ EcoR1 | |
Colony Screening
[edit]Date: 2012-4-10
Make miniprep and digest with Nde1+ EcoR1
clone 1,2,4,8 may be right
c
Result intepretation/Summary
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Conclusion
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