496-pAd52-CMV5-Cuo-MCS3
Cloning of 496-pAd52-CMV5-Cuo-MCS3
[edit]Purpose: Cloning of 496-pAd52-CMV5-Cuo-MCS3
Date: 2012-4-23---2012-4-25
Notebook:0
Page:
Clone ID:496
Experimenters: yingying
Results
[edit]cloning
[edit]
| band 1 | ||
| pAd52-CMV5-MCS 495 | ||
| enzymes |
EcoR11 + Sal1 | |
digest pAd52-CMV5-MCS 495 with EcoR11 + Sal1, ligate to annealed pAd52-CMV5-MCS3-T + pAd52-CMV5-MCS3-B
Colony Screening
[edit]Date: 2012-4-25
Make miniprep and digest with Xho1+ Kpn1 and HindIII + Pst1
clone 3 may be right
Result intepretation/Summary
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Conclusion
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