1080-pBad24-RNAPol
Cloning of 1080-pBad24-RNAPol
[edit]Purpose: Cloning of 1080-pBad24-RNAPol
Date: 2012-4-20---2012-4-25
Notebook:0
Page:
Clone ID:1080
Experimenters: yingying
Results
[edit]cloning
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PCR using 1method: T7 phage DNA; 2method: Nico21(DE3) cell genomic DNA as template.
T7RNAPol-EcoR1-F
EcoR1
acgaca GAATTC ATG AACACGATTAACATCGCTAAGAAC
T7RNAPol-HindIII-R
(GAGTCGGACTTCGCGTTCGCG CAT CAT CAT CAT CAT CAC TAA AAGCTT atacta)
| band 1 |
band 2 | |
| PCR product |
pBad24 545 | |
| enzymes |
EcoR1 + HindIII | |
Colony Screening
[edit]Date: 2012-4-25
Make miniprep and digest with EcoR1 + HindIII
Result intepretation/Summary
[edit]- <br>
Conclusion
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