1135 p8H

Digest pET28b with Nco1 + Nde1

ligate to annealed
Nco1-8XHis-Nde1-T
Nco1-8XHis-Nde1-B

5/11/2012

Sequencing result: #1 is no good (both Nde1 + Nco1 sites are gone, which means the oligos are not annealed properly), so digest plasmid DNA with Nde1 + Sma1. Send clone #3 for sequencing

3/12/2012: Sequencing result: #3 is good. Both Nco1 and Nde1 are preserved, and 8XHis tag is added to the vector.