1092-pSecTag3
Cloning of 1092-pSecTag3
[edit]Purpose: Cloning of 1092-pSecTag3
Date: 2012-4-17---2012-4-19
Notebook:0
Page:
Clone ID:1092
Experimenters: yingying
Results
[edit]cloning
[edit]digest pSecTag-C-Fc-MEA 354 with BamH1 + Xho1,--> vector
ligate to annealed SecTag-MCS-T + SecTag-MCS-B
| band 1 | |
| pSecTag-C-Fc-MEA 354 | |
| enzymes |
BamH1 + Xho1 |
Colony Screening
[edit]Date: 2012-4-19
Make miniprep and digest with Nhe1
not the right band
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
<br>
<br>
Cloning of 1092-pSecTag3
[edit]Purpose: Cloning of 1092-pSecTag3
Date: 2012-4-26---2012-4-30
Notebook:0
Page:
Clone ID:1092
Experimenters: yingying
Results
[edit]cloning
[edit]digest pSecTag-C-Fc-MEA 354 with BamH1 + Xho1,--> vector
ligate to annealed SecTag-MCS-T + SecTag-MCS-B
| band 7 | |
| pSecTag-C-Fc-MEA 354 | |
| enzymes |
BamH1 + Xho1 |
Colony Screening
[edit]Date: 2012-4-30
Make miniprep and digest with Nhe1
no insert
Result intepretation/Summary
[edit]- &lt;br&gt;
Conclusion
[edit]&lt;br&gt;
&lt;br&gt;
<br>
<br>
Cloning of 1092-pSecTag3
[edit]Purpose: Cloning of 1092-pSecTag3
Date: 2012-5-9----2012-5-14
Notebook:0
Page:
Clone ID:1092
Experimenters: yingying
Results
[edit]cloning
[edit]digest pSecTag-C-Fc-MEA 354 with BamH1 + Xho1,--> vector
ligate to annealed SecTag-MCS-T + SecTag-MCS-B
| band 1 | |
| pSecTag-C-Fc-MEA, 354 | |
| enzymes |
BamH1 + Xho1 |
Colony Screening
[edit]Date: 2012-5-14
Make miniprep and digest with Nhe1
not the right band
Result intepretation/Summary
[edit]- &lt;br&gt;
Conclusion
[edit]&lt;br&gt;
&lt;br&gt;
<br>
<br>