1129 pDB.His.PfuMBP
May 2, 2012
PCR using template from gene synthesis
digest with Kpn1 + Nde1, clone into Kpn1+ Nde1 digested 360 pDB.His.MBP
Select 6 clones to mini-prep
1129 plasmids are digested by Nde1 + Kpn1
Select 1129-5 to sequence.
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[edit]
Cloning of 1129 pDB.His.PfuMBP
[edit]Purpose: Cloning of 1129 pDB.His.PfuMBP
Date: 2012-5-17---
Notebook:0
Page:
Clone ID:1129
Experimenters: yingying
Results
[edit]cloning
[edit]
| band 1 |
band 2 | |
| PCR product |
360 pDB.His.MBP | |
| enzymes |
Kpn1 + Nde1 | |
2012-5-18
| band 3 |
band 4 | |
|
PCR product |
360 pDB.His.MBP | |
| enzymes |
Kpn1 + Nde1 | |
Colony Screening
[edit]Date: 2012-5-21
Make miniprep and digest with Kpn1 + Nde1
clone 1,2,3 need to sequence
Result intepretation/Summary
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Conclusion
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