212-pDBHisMBP-TthMuts
Cloning of 212-TthMuts
[edit]Purpose: Cloning of TthMuts by PCR
Date:
Notebook:
Page:
Clone ID: 212
Experimenter: Jennifer Truong
Results
[edit]Cloning
[edit]Date: 5/24/2012
Notebook/page:
PCR cloning using genomic DNA.
| Lane |
1 |
2 |
3 |
| Sample (Genomic DNA) |
HP8 |
HP27 |
HP27 |
| Sample Lot |
57761360 |
4728857 |
Cut out the ~2.4 kB band, add gel solubilization buffer. Purify and digest with Nde1 + EcoR1. Digest pDB.His.MBP with Nde1 + EcoR1.
5/27/2012
digest miniprep with Nde1+ EcoR1.
not good
PCR Screening
Samples 1-8
Possibly success in lane 3, but unclear.
There is no positive band for these samples (No 9 to 16 PCR samples)
No positive results
Experiment abandoned because there were no positive results from PCR
Result intepretation/Summary
[edit]Experiment abandoned because there were no positive results from PCR screening.
Conclusion
[edit]| Experiment abandoned because there were no positive results from PCR screening. |