1190 pCDNA3.1-Dest

5/20/2012

1: 374 pAd5-CMV-Dest-C6XHis

2: 524 pCDNA3.1-E1A

3: 335 pCDNA3.1

digest with Kpn1 + Xho1.

digest 374 pAd5-CMV-Dest-C6XHis with Kpn1+ Xho1--->gateway cassette
Digest pCDNA3.1, 335 with Kpn1 + Xho1 -->vector
ligate and transform ccdb survival

digest miniprep DNA with Kpn1+Xho1

Clone #4 is good, digest with Nco1 + Not1, Not1 + BamH1, and BamH1.

	band 1	band 2	band 3
	Nco1+Not1	Not1+BamH1	BamH1
Expected Size	621	773	703

Clone #4 has correct restriction sites.

5/28/2012

1	2	3	4
Not1+ BamH1	Nco1+Not1	EcoR1+Pst1	BamH1

5/29  1190 are digested by Kpn1+Xho1

pHis9 digest with Not1 + BamH1

6/9/2012

Digest new 1190 pCDNA3.3-Des with Kpn1 + Xho1, or Not1 + BamH1