1225 pET28b-T7-Endo1
Cloning of T7 endonuclease 1, gene 3 into pET28b
[edit]Purpose: Cloning of T7 endonuclease 1, gene 3 into pET28b
Date: 06/08/2012
Notebook:
Page:
Clone ID: 1225
Experimenters: Jennifer Truong
Cloning
[edit]06/08/2012: Amplified gene with PCR (KAPA enzyme, 20 cycles) Cut out circled bands for purification and digestion
Digested purified DNA and pET28b vector with Nde1 and Xho1, cut out bands for ligation (lanes 3 and 4)
Colony Screening
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DNA very weak, submitted sample #1 (lane 7) for sequencing
Result intepretation/Summary
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Conclusion
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