Expression Systems

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E.coli (soluble/refolding)
• Homogeneity, labeling
• Complexity, modifications
• Time: 1-2 w; mg-g/L
Purification/
Analytics
Refolding
• Time: 1-14 days
Tag purification
• Time: 1-3 days
BEVS (Insect-Baculo)
• Modifications
• Secretion, degradation
• Time: 4-6 w; ~ 5 mg/L
DES (Insect-Drosophila)
Native Purification
• Time: 1-3 days
Analytics
• Modifications, scalable
• Secretion, degradation
• Time: 4-6 m; ~ 5-30 mg/L
Mammalian Cells
• Modifications, secretion
• Large amounts of pDNA
• Time: 1-3 w; ~ low mg/L

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