1245 pDB.His.MBP.scIHF2
Cloning of 1245 pDB.His.MBP.scIHF2
[edit]Purpose: Cloning of 1245 pDB.His.MBP.scIHF2
Date: July 6, 2012
Notebook:
Page:
Clone ID: 1245
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Amplified from 1078 template with scIHF-Nhel-F and scIHP-2-16 primers, expected size 620 bp
Cut out band from lane 3, purified digested with 1217 vector with RE Nhe1 and Xho1
Digested DNA with 1217 vector with RE Nhe1 and Xho1
Cut out bands from lane 1 and 2 (pDB.His.MBP ?), purify and ligate together
Ligate and and transform, plate and let grow overnight
Colony Screening
[edit]Date: June 9, 2012
Digested MiniPrep plasmids with restriction enzymes Nhel and Xhol, positive inserts for samples 1, 2, 3 and 4