1222-pCMV5-Fc(3C)-Dest

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Cloning of 1222-pCMV5-Fc(3C)-Dest

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Purpose: Cloning of 1222-pCMV5-Fc(3C)-Dest

Date: 2012-7-10---2012-7-17

Notebook:0

Page:

Clone ID:1222

Experimenters: yingying

Results

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subcloning

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2012-7-9



 lane 1
 lane 2

 448 pLex-CMV5-CuO
 1223 pCDNA3.3-Fc(3C)-Dest
enzymes
       Nde1 + BamH1



Colony Screening

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Date: 2012-7-12

Make miniprep and digest with Nde1 + BamH1

clone 3,4,5,6,7,8 may be right


2012-7-17

clone 3 digest detect:

lane 1:Nde1+BamH1

lane 2:Nde1+Hind111

clone 3 right

Result intepretation/Summary

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  • <br>

Conclusion

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<br>







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