1247-pCDNA3.1-PBasic-PH

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Cloning of pCDNA3.1-PBasic-PH(1247)
Purpose: to clone pCDNA3.1-PBasic-PH
Date: 2012-7-13 to 2012-7-19
Notebook: Xiaojing Wang
Page: 005296

Clone ID: 1247
Experimenters: Xiaojing Wang

Results

synthesize pBasic + PH

1247 PCR 7.16.2012.JPG

Digest with Xho1 + BamH1, ligate to Xho1 + BamH1 digested pCDNA3.1, 1226


Transform DH5alpha

Pick up 6 clones to mini-prep
1247 plasmids are digested by Xho1 + BamH1


Clone 1247-1,-2,-3,-4,-5,-6 may be correct.

Pick up 1247-2 to sequence.


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