1163 pFUSE2-aKOD-CLIg-hk
Cloning of 1163 pFUSE2-aKOD-CLIg-hk
[edit]Purpose: Cloning of 1163 pFUSE2-aKOD-CLIg-hk
Date: 2012-6-1
Notebook: 0
Page:
Clone ID: 1163
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: 6/1/2012
Synthesized template by gene synthesis, cut out band for cloning next week
Date: July 19, 2012
Digested DNA (lane 1) and pFUSE2-CLIg-hk,1162 lane 2) with restriction enzymes AgeI + Nco1
Not a clear vector band, may need to redigest tomorrow. There is no fast digest AgeI, used NEB enzyme
Cut out DNA band and saved in box
Colony Screening
[edit]Result intepretation/Summary
[edit]