Batch Medium

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Component Final concentration [g/L], mg/L] and [ml/L] of purified water
(NH4)2SO4 14.0
Glycerol 35.0
Yeast extract (Becton Dickinson) 20.0
KH2PO4 2.0
K2HPO4 16.5
Citric acid 7.5
MgSO4.7H2O 2.47
H3PO4 1.5 ml/L
CaCl2.2H2O 0.294
Antifoam AF204 0.2 ml/L
Tetracycline 15 mg/L
FeSO4.7H2O 114 mg/L
ZnSO4.7H2O 29 mg/L
MnSO4.H2O 17 mg/L
Na2MoO4.2H2O 9 mg/L
CuSO4.5H2O 4 mg/L
H3.BO3 12 mg/L

Glycerol/Magnesium chloride feed
Component of Feed Amount required [g/L] of purified water
Glycerol 714
MgSO4.7H2O 7.4

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